G-Sep™ Phenyl Agarose 6 Fast Flow (High Sub), 25ml, 786-959, G-BIOSCIENCES (CAT#: STEM-C-3285-LGZ)

G-Sep™ Phenyl Sepharose Fast Flow (FF) is 6% cross-linked agarose with phenyl hydrophobic interaction chromatography (HIC). This resin is used to separate biomolecules on the basis of relative hydrophobicity.
G-Sep™ Phenyl Sepharose Fast Flow is available in two levels of ligand substitution to help find the optimum selectivity and binding capacity for a given application.
G-Sep™ Agarose for HIC can also immobilize butyl and octyl groups on 6% cross-linked agarose beads via neutral and highly stable ether linkages.

Size: 25ml
Matrix: Highly cross-linked Agarose, 6%
Bead form: Spherical, diameter 50-160μm
Ligand: Phenyl
Ligand Concentration: About 25μmol/ml or 40μmol/ml for low and high sub respectively
Binding Capacity: About 20mg HSA/ml resin or 20mg HSA/ml resin for low and high sub respectively
pH stability Working Range: 3-13
pH stability Cleaning-in-Place (CIP): 2-14
Maximum Pressure (MPa): 0.3
Maximum Flow Velocity: 450cm/h
Exclusion Limit (Globular Proteins): 4 x 106
Physical Stability: Negligible volume variation due to changes in pH or ionic strength
Chemical Stability: Stable to all commonly used aqueous buffers: 1 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol
Sterilization: Autoclavable, In 1M NaOH,121 °C, pH 7, for 30 min
Storage Conditions: 4 to 30°C, 20% Ethanol

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