G-Sep™ SP Agarose Fast Flow, 300ml, 786-971, G-BIOSCIENCES (CAT#: STEM-C-3305-LGZ)

G-Sep™ SP Sepharose Fast Flow (FF) Resin is a strong cation exchanger consisting of highly cross-linked 6% agarose beads with thiopropyl (SP) strong cation exchange groups. This resin is widely used in the separation of biomacromolecules in research and industrial applications. G-Sep™ SP Sepharose Fast-Flow (FF) Resin has high chemical stability, allows for well-validated cleaning-in-place (CIP) and sanitization protocols, and has become the industry standard for ion-exchange chromatography in recent decades.

Size: 300ml
Matrix: Cross-linked agarose beads, 6%
Ligand: Sulphopropyl
Ion Exchanger : Strong cation exchanger
Bead form: Spherical, diameter 45-165μm
Ionic Capacity: 0.18-0.25 mmol (Na+)/ml
Binding Capacity: 70 mg lysozyme /ml medium
pH stability Working Range: 2-12
pH stability Cleaning-in-Place (CIP): 2-14
Maximum Flow Velocity: 450cm/h
Maximum Pressure: 0.3MPa
Exclusion limit(globular proteins): 4 x 106
Physical Stability: Negligible volume variation due to changes in pH or ionic strength
Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8M urea, 8M guanidine hydrochloride, 70% ethanol
Autoclavable: With Na+ as counter Ions, at 121°C, pH 7, for 30 min in 0.2M sodium acetate for autoclaving
Storage Conditions: 4 to 30°C, 20% Ethanol containing 0.2M sodium acetate

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