G-Trap™ Phenyl Agarose 6 Fast Flow (High Sub), 5 ml, 5 Columns, 786-1006, G-BIOSCIENCES (CAT#: STEM-C-3296-LGZ)

G-Trap™ Phenyl Sepharose 6 Fast Flow (High Sub) is prepackaged for the separation of biomolecules by hydrophobic interaction chromatography (HIC).

Biomolecules are separated on HIC columns according to the hydrophobicity of the ligands attached to the resin, the distribution of surface-exposed hydrophobic amino acids on the protein or enzyme to be separated, and the concentration and type of salt used for binding or priming buffers. In HIC, the binding of biomolecules to the column (ligand) is facilitated by using high concentrations of anti-orientation salts, such as ammonium sulfate, sodium sulfate, etc., in the binding buffer or initiation buffer.

Straight alkyl chain ligands (such as butyl, octyl) exhibit purely hydrophobic properties, aryl ligands (such as phenyl) exhibit mixed modes, and aromatic, hydrophobic, and uncharged play a role on protein/biomolecule adsorption columns. effect.

G-Biosciences offers a wide range of G-Trap™ HIC columns, including G-Trap™ Butyl Sepharose 6 Fast Flow, G-Trap™ Octyl Sepharose 6 Fast Flow, G-Trap™ Phenyl Sepharose 6 Fast Flow (High Sub), G-Trap™ Phenyl Sepharose 6 Fast Flow (Low Sub), so that one can choose a HIC column suitable for separating the target protein. In addition, G-Trap™ HIC Selection Kit can be used for preliminary screening of appropriate hydrophobic columns and purification conditions for target proteins

Size: 5 columns

G-Trap™ Phenyl Agarose 6 Fast Flow (High Sub) Resin

Mean particle size: 90 µm
Bead structure: Highly cross-linked 6% Agarose
Ligand: Phenyl
Ligand Concentration: About 40μmol/ml
Binding Capacity: About 40mg HSA/ml resin
pH stability Working Range: 3-13
pH stability Cleaning-in-Place (CIP): 2-14
Maximum Pressure (MPa): 0.3
Maximum Flow Velocity: 450cm/h
Exclusion Limit (Globular Proteins): 4 x 106
Physical Stability: Negligible volume variation due to changes in pH or ionic strength
Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol
Storage Conditions: 4 to 30°C, 20% Ethanol

G-Trap™ Phenyl Agarose 6 Fast Flow (High Sub), 1 ml columns

Column Volume: 1 ml
Columns Dimension: 0.7 x 2.5 cm
Column Harware Pressure Limit: 0.5 MPa
Column Hardware: Polypropylene (Biocompatible)

G-Trap™ Phenyl Agarose 6 Fast Flow (High Sub), 5 ml columns

Column Volume: 5 ml
Columns Dimension: 1.6 x 2.5 cm
Column Harware Pressure Limit: 0.5 MPa
Column Hardware: Polypropylene (Biocompatible)

FastPure™ Mini Spin Column Pack, 600 µl, 100 Columns, 786-1090, G-BIOSCIENCES (CAT#-STEM-C-3319-LGZ)
FastPure™ Midi Spin Column Pack, 20 ml, 8 Columns, 786-1091, G-BIOSCIENCES (CAT#-STEM-C-3320-LGZ)
Spin Column, 3ml, 25 Columns, 786-724, G-BIOSCIENCES (CAT#-STEM-C-3321-LGZ)
Spin Column, 3ml, 50 Columns, 786-725, G-BIOSCIENCES (CAT#-STEM-C-3322-LGZ)
Spin Column, 5ml, 10 Columns, 786-726, G-BIOSCIENCES (CAT#-STEM-C-3323-LGZ)
Spin Column, 5ml, 25 Columns, 786-981, G-BIOSCIENCES (CAT#-STEM-C-3324-LGZ)