G2229A LC, Agilent Technologies (CAT#: STEM-CPO-0538-ZJF)

The manufacturer of the G2229A LC, Agilent Technologies is Agilent Technologies.
Refurbished Agilent 1100 Series Nanoflow LC bundle for MS (Agilent G2229A) complete with nano-LC pump (G2226A), on-line micro vacuum degasser (G1379A), micro monowell- micro well-plate autosampler (G1377A) and 1100 Series handheld controller (G1323B).
Optimum solution to work at stable, precise nanoliter flow rates in combination with nanoelectrospray for highest sensitivity.

Condition: Refurbished, Certified Pre-owned
Specifications for nanoflow pump:
Settable flow range 0.01-4 μL/min, 1-2500 μL/min (with EFC bypassed)
Recommended flow range 0.1-1 μL/min, 200-2500 μL/min (with EFC bypassed)
Flow precision < 0.7 % RSD (typically < 0.4 % RSD) at 600 nL/min (BSA digest)
Delay volume Typically 300 nL from EFC to pump outlet for flow rates up to 4 μL/min
Specifications for micro vacuum degasser:
Maximum flow rate 10 mL/min per channel
Number of channels 4
Internal volume Typically 1 ml per channel
Material in contact with solvents PTFE, FEP, PEEK
Specifications for micro well-plate autosampler:
Sample capacity 2 well plates (96 and 384) plus 10 additional 2-ml vials 108 × 2-ml vials in two 54-vial plates
Settable injection volume: 0.01–8 μl with small loop capillary; 0.01–40 μl with extended loop capillary
Precision: Typically: < 0.5 % RSD from 5–40 μl,< 1 % RSD from 1–5 μl, < 3 % RSD from 0.2–1 μl
Carryover: Typically < 0.05 % using the following conditions:
Column: 150 x 0.5 mm, Hypersil ODS, 3 μm.
Mobile phase: water/ acetonitrile = 85/15.
Column flow rate: 13 μL/min
Injection volume: 1μL caffeine (= 25ng caffeine)1 μL water to test carryover.
Outside wash of needle: 20 sec with water using flush port

2D LC system- 2nd dimension LC system.
LC system used for mass spectrometry (MS)-based proteomics applications
Nanoflow settable flow range: 0.01-4 microL/min, 1-2500 microL/min
Nanoflow flow precision: <0.7% RSD at 600 nL/min
Micro vacuum degasser maximum flow rate: 10 mL/min per channel

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