His60 Ni Magnetic Beads, 10 mL, 635692, Takara Bio Inc (CAT#: STEM-C-3847-LGZ)

Highlights

Choose from native or denaturing conditions to purify proteins
Directed expression of 6xHis-tagged proteins improves reproducibility and signal-to-noise ratio
Different numbers of beads per well allow for a wide range of binding capacities

Cat Number: STEM-C-3847-LGZ

Application: His60 Ni Magnetic Beads provide simple, effective separation of recombinant his-tagged proteins for a wide range of applications, including:<br /><br />Microscale purification of his-tagged proteins<br />Studying protein structure and function<br />Preparing proteins for X-ray crystallography<br />Performing assays that detect protein-protein and protein-DNA interactions<br />Immunization to raise antibodies against a protein of interest<br />Screening for protein expression<br />Optimizing purification conditions for scale-up with His60 Ni Resin<br />His60 Ni Magnetic Beads can also be used to immobilize 6xhis-tagged proteins for affinity chromatography, in order to:<br /><br />Analyze protein-protein and protein-nucleic acid interactions<br />Purify untagged subunits or nucleic acids that interact with the immobilized protein<br />Perform antibody purification<br />Study interactions between ligands and receptors

Model: 635692