His60 Ni Superflow Resin, 250 mL, 635662, Takara Bio Inc (CAT#: STEM-C-3821-LGZ)

His60 Ni Superflow Resin is a high capacity Ni-IDA resin for the one-step purification of recombinant his-tagged proteins from bacterial, mammalian, and baculovirus-infected cells. The modified Ni-IDA chemistry makes this resin one of the best on the market in terms of capacity, metal ion leakage and purity when compared to other nickel-based resins such as Ni-NTA or other Ni-IDA resins. See how His60 outperforms Ni-NTA.

Purification principle of recombinant his tagged protein

His60 Ni superfluid resin is compatible with batch/gravity flow applications, as well as major automated LC systems and manual syringe handling. The resin has low metal ion leakage and the highest binding capacity to date (better than Ni-NTA and other resins). The resin allows for fast, simple, reproducible chromatographic separations and can be regenerated for multiple uses.

Purification process of recombinant his-tagged protein

The purification of recombinant his-tagged protein consists of 4 steps: cell lysis/extraction, binding, washing and elution. His60 Ni superfluid resin allows one-step protein purification under native or denaturing conditions. This resin is compatible with a wide variety of denaturants and detergents, see user manual for a complete reagent compatibility table. Purified recombinant His-tagged proteins can be eluted by adding 300 mM imidazole or lowering the pH.

Size: 250 mL

Up to 60 mg/ml recombinant his-tagged protein
Low leakage of metal ions in the elution of his-tagged proteins
For purification under native or denaturing conditions
For batch/gravity flow and automated FPLC cleanup

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