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PROTEINDEX™ PureBind™ Co-NTA Agarose 6 Fast Flow, Prepacked Cartridge, 5 x 5 mL, 11-0253-5x5ML, Marvelgent biosciences (CAT#: STEM-C-3680-LGZ)

Highlights

Ready-to-use, convenient formats: 1ml and 5ml.
Good mechanical stability, suitable for the preparation and large-scale purification of histidine-tagged proteins.
Compared with nickel-based IMAC, his-tagged protein has higher specificity and selectivity. Can be used to purify challenging proteins that are expressed at low levels or when nickel is not required.

Cat Number: STEM-C-3680-LGZ

Application: For small-scale and preparative large-scale purification of histidine-tagged recombinant proteins from prokaryotic and eukaryotic expression systems.

Model: 11-0253-5x5ML

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Description

PROTEINDEX™ Co-NTA Sepharose 6FF is a cobalt-based immobilized metal ion affinity chromatography (IMAC) medium. Compared to nickel-containing media, it offers greater specificity and therefore increased selectivity for polyhistidine-tagged proteins under both native and denaturing conditions. Co-NTA Sepharose 6 Fast Flow Resin can be used for small-scale and preparative large-scale purification of histidine-tagged recombinant proteins in prokaryotic and eukaryotic expression systems.

Specification

Size: 5 x 5 mL
Ligand: Cobalt ion
Binding capacity: >20 mg 6XHis-tagged protein/mL medium
Matrix: Highly cross-linked 6% agarose resins
Particle size: 45 μm - 165 μm
Maximum pressure: 0.3 MPa (3 bar)
Storage temperature: 2 - 8°C
Storage buffer: 1X PBS containing 20% ethanol

Features

Ready-to-use, convenient formats: 1ml and 5ml.
Good mechanical stability, suitable for the preparation and large-scale purification of histidine-tagged proteins.
Compared with nickel-based IMAC, his-tagged protein has higher specificity and selectivity. Can be used to purify challenging proteins that are expressed at low levels or when nickel is not required.
Suitable for protein purification in prokaryotic and eukaryotic expression systems.
Compatible with applications under native and denaturing conditions.
Low cobalt ion leakage.

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