HPP-60(High Density) Phenyl Hydrophobic Media (High Density), Highly Cross-Linked Polyacrylate Microspheres, Bogen (CAT#: STEM-C-3049-LGZ)

Hydrophobic chromatography utilizes the adsorption of proteins on the hydrophobic groups of the chromatography medium at high salt concentration. When the salt concentration is reduced, different proteins are eluted in order from weak to strong hydrophobicity, so as to achieve the purpose of separation and purification. With mild operating conditions and high resolution, hydrophobic chromatography is one of the most commonly used methods for the separation and purification of biological macromolecules. The HP series of hydrophobic chromatography media developed by Bogen are based on polyacrylate microspheres with high cross-linking degree and Butyl, Octyl, Phenyl, etc. as ligands (the hydrophobicity increases in turn). Isolation and purification of plasmids, etc.

Matrix: highly cross-linked polyacrylate microspheres
Average particle size: 70±10μm
Average pore size: 30nm
Functional groups and ligand density
Butyl: 30 μmol/ml wet gel
Octyl: 5 μmol/ml wet gel
Phenyl: 20 μmol/ml wet gel
Maximum flow rate (25°C): 1000 cm/h
Maximum pressure: 1 MPa (10 bar)
pH stability: 2~13 (long time); 1~14 (short time)
Chemical stability: 1M NaOH, 0.01M HCl, 6M guanidine hydrochloride, 8M urea soaked for 7 days at room temperature, no obvious change in performance
Physical stability: The pH or ionic strength of the solution affects the volume change rate of the medium <0.1%
Storage: 4~30 °C (20% ethanol)

The medium retains the high mechanical strength of the polymer microspheres while having good compatibility with bioactive macromolecules.

GPP-60(Low Density) Phenyl Hydrophobic Media (Low Density), Highly Cross-Linked Polyacrylate Microspheres, Bogen (CAT#-STEM-C-3059-LGZ)
CPB-30(High Density) Butyl Hydrophobic Media (High Density), Polyacrylate Microspheres, Bogen (CAT#-STEM-C-3060-LGZ)
CPB-60(High Density) Butyl Hydrophobic Media (High Density), Polyacrylate Microspheres, Bogen (CAT#-STEM-C-3061-LGZ)
CPB-30(Low Density) Butyl Hydrophobic Media (Low Density), Polyacrylate Microspheres, Bogen (CAT#-STEM-C-3062-LGZ)
CPB-60(Low Density) Butyl Hydrophobic Media (Low Density), Polyacrylate Microspheres, Bogen (CAT#-STEM-C-3063-LGZ)
CPP-30(High Density) Phenyl Hydrophobic Media (High Density), Polyacrylate Microspheres, Bogen (CAT#-STEM-C-3064-LGZ)