Imaging of Chromatin Structure in Pathological Tissue by Stochastic Optical Reconstruction Microscopy (CAT#: STEM-MIT-0375-LJX)

Introduction

Chromatin is the material in the nucleus that can be stained with basic dyes during the intercellular phase. The basic chemical component of chromatin is deoxyribonucleoprotein, which is a complex of DNA, histone, non-histone, and a small amount of RNA. It is the form in which the genetic material of interphase cells exists. Direct visualization of higher-order chromatin structure at the molecular scale is of great importance for understanding the impact of chromatin organization on gene expression in many biological processes.

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Principle Applications Procedure Materials Notes Related Products

Principle

Principles of stochastic optical reconstruction microscopy: By fitting the two-dimensional Gaussian function to determine the centroid of microscope-formed light spots, a single fluorescent source (such as a fluorescent group) can be located with high precision. The accuracy of the calculation to determine the centroid depends only on the number of photons collected, and the resolution scale can be tens of nanometers or smaller. To achieve this accuracy, the density of the fluorescent molecules being tested is required to be low enough that the spots of the two fluorescent groups are unlikely to overlap.

Applications

Imaging in two or three dimensions, in multiple colors, and even in living cells
Applied in many areas of the life sciences, and provides very high resolution images for many different needs from neuroscience to subcellular science

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Chromatin structure in pathological tissue

Notes

Operate in strict accordance with the operating procedures, and shall not arbitrarily change the operating procedures

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