Imaging of living brain tissue by stimulated emission depletion microscopy (CAT#: STEM-MIT-0352-LJX)

Introduction

As the most advanced part of the nervous system, the brain dominates all activities in the body and regulates the balance between the body and the surrounding environment. It is the material basis of higher nervous activities.

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Principle Applications Procedure Materials Notes Related Products

Principle

Stimulated emission depletion (STED) microscopy uses two light sources. One source emits light that excites the fluorophores, and the other emits a ring laser of different wavelengths, which is used to suppress fluorescence.

Applications

Imaging of the intensity distribution of the fluorescent sample
Imaging of living samples
Measuring of the fluorescence lifetime and fluorescence correlation spectrum of the fluorescent samples
Used in the fields of biology, medicine and materials science

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Living brain tissue

Notes

Operate in strict accordance with the operating procedures, and shall not arbitrarily change the operating procedures

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