Imaging of Clathrin-Mediated Endocytosis in Living Cells by Stimulated Emission Depletion (STED) (CAT#: STEM-MIT-0351-LJX)

Introduction

Clastrin-mediated endocytosis is a process by which cells bud inward through the plasma membrane (invagination) to absorb metabolites, hormones, proteins, and certain viruses. This process forms vesicles containing the absorbed substance and is strictly mediated by receptors on the cell surface. Only receptor-specific substances enter cells through this process.
Endocytic events on the plasma membrane of living cells can be imaged using stimulated emission reduction microscopy.

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Principle Applications Procedure Materials Notes

Principle

Stimulated emission depletion (STED) microscopy uses two light sources. One source emits light that excites the fluorophores, and the other emits a ring laser of different wavelengths, which is used to suppress fluorescence.

Applications

Imaging of the intensity distribution of the fluorescent sample
Imaging of living samples
Measuring of the fluorescence lifetime and fluorescence correlation spectrum of the fluorescent samples
Used in the fields of biology, medicine and materials science

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Living plasma membrane

Notes

Operate in strict accordance with the operating procedures, and shall not arbitrarily change the operating procedures

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