Imaging of RNA- and protein-containing domains in fixed cells by stimulated emission depletion microscopy (CAT#: STEM-MIT-0350-LJX)

Introduction

A domain is a level between the super-secondary and tertiary structures. A domain is an individual folded unit within the tertiary structure of a protein, usually a combination of several super-secondary units.
Super resolution microscopy has achieved to visualize spatial arrangements of RNA- and protein-containing domains in individual cells.

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Principle Applications Procedure Materials Notes Related Products

Principle

Stimulated emission depletion (STED) microscopy uses two light sources. One source emits light that excites the fluorophores, and the other emits a ring laser of different wavelengths, which is used to suppress fluorescence.

Applications

Imaging of the intensity distribution of the fluorescent sample
Imaging of living samples
Measuring of the fluorescence lifetime and fluorescence correlation spectrum of the fluorescent samples
Used in the fields of biology, medicine and materials science

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
A novel lncRNA, Tumor-associated NBL2 transcript (TNBL)

Notes

Operate in strict accordance with the operating procedures, and shall not arbitrarily change the operating procedures

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