Imaging with the HyPer2 Biosensor by Stimulated Emission Depletion Microscopy (CAT#: STEM-MIT-0366-LJX)

Introduction

Biosensor is an analytical device composed of biosensors and signal transducers. Stimulated emission depletion (STED) microscopy is a popular super resolution imaging technique. Not only synthetic dyes and fluorescent proteins can be utilized as STED fluorophores, but also genetically encoded biosensors. Fusing the biosensor with proteins of interest allows subdiffraction imaging of intracellular macromolecular architecture with simultaneous extraction of functional information about cellular activities. The service provides a protocol for live-cell STED microscopy of the HyPer2 biosensor fused to cytoskeletal filaments.

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Principle Applications Procedure Materials Notes Related Products

Principle

Stimulated emission depletion (STED) microscopy uses two light sources. One source emits light that excites the fluorophores, and the other emits a ring laser of different wavelengths, which is used to suppress fluorescence.

Applications

Imaging of the intensity distribution of the fluorescent sample
Imaging of living samples
Measuring of the fluorescence lifetime and fluorescence correlation spectrum of the fluorescent samples
Used in the fields of biology, medicine and materials science

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
HyPer2 biosensor

Notes

Operate in strict accordance with the operating procedures, and shall not arbitrarily change the operating procedures

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