Imaging of dendritic spines in living hippocampal slices by stimulated emission depletion microscopy (CAT#: STEM-MIT-0354-LJX)

Introduction

Dendrites are short, branching protrusions from the cell body of a neuron. The dendrite receives impulses from other neurons, so its range is representative of the range of stimuli received by that neuron.

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Principle Applications Procedure Materials Notes

Principle

Stimulated emission depletion (STED) microscopy uses two light sources. One source emits light that excites the fluorophores, and the other emits a ring laser of different wavelengths, which is used to suppress fluorescence.

Applications

Imaging of the intensity distribution of the fluorescent sample
Imaging of living samples
Measuring of the fluorescence lifetime and fluorescence correlation spectrum of the fluorescent samples
Used in the fields of biology, medicine and materials science

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Dendritic spines in living hippocampal slices

Notes

Operate in strict accordance with the operating procedures, and shall not arbitrarily change the operating procedures

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