G-Biosciences Immobilized Heparin is a purification resin that can be used for a variety of proteins. The resin consists of 6% cross-linked agarose covalently coupled to heparin via an amide bond. The coupling chemistry used results in a highly stable purified resin, the most commonly used stabilizing buffers and denaturants.
Heparin is a linear glycosaminoglycan composed of equimolar amounts of glucosamine and glucuronic acid, linked alternately by α(1→4) glycosidic bonds. Some of its hydroxyl groups are esterified with sulfate groups, and the molecule has a reducing sugar end.
Due to its structure and biochemical role, heparin is able to bind many proteins, enzymes and polycationic organic compounds. Binding is an ionic or more specific protein-ligand or enzyme-inhibitor (or enzyme-activator) interaction.
There are several classes of proteins that bind heparin, including:
Lipoprotein lipase: through ionic interactions Lipoproteins: LDL, VLDL, VLDL Apo, HDL Growth hormone Growth factors: FGF, ECGF DNA and RNA-related enzymes: polymerases, nucleases, endonucleases Enzymes: including collagenase, hyaluronidase, lysozyme, protease