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In this paper, pH-sensitive zein/gelatin (ZG) fiber membranes incorporated with curcumin (CUR) and/or alizarin (ALI) were prepared by electrospinning. The pH sensitivity of each ZG fiber membrane was analyzed. The influences of different pigments on the morphology, structure, heat resistance and water resistance of ZG fiber membrane were compared by scanning electron microscopy (SEM), infrared spectroscopy (FTIR), X-ray diffraction (XRD), differential scanning calorimetry (DSC) and water contact angle (WCA). Furthermore, the freshness indicator effect of different ZG fiber membranes on tuna fillets stored at 4 % was evaluated.