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This service provides a novel inhibition assay by capillary electrophoresis/dynamic frontal analysis (CE/DFA). When substrate p-nitrophenyl phosphate and inhibitor theophylline were tandemly introduced into a capillary containing alkaline phosphatase enzyme, two plateau signals were detected in the electropherogram. A higher plateau is based on the CE/DFA without inhibition, and a suppressed plateau is formed while the substrate zone passes through the inhibitor zone. Inhibition constant was successfully determined through the two plateau heights.