Isolation of Capsaicin and Dihydrocapsaicin for Vegetable Oil Adulteration (CAT#: STEM-ACM-0111-CJ)

Introduction

Waste oil, consisting of cooking oil collected from food wastes, waste cooking oils recycled from drains or grease traps, residual oils extracted from animal fats, deep frying oils, and other inedible oils, have caused serious food safety scandals in some countries when they are illegally sold and used as edible oils. These inedible oils are generally contaminated by hazardous compounds such as condiments, heavy metal ions, bio-toxins, polycyclic aromatic hydrocarbons (PAH), 3-monochloropropane-1,2-diol (3-MCPD) and its esters, phthalates, dioxins and polychlorinated biphenyls (PCBs) or degradation products of oxidation process.

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Principle Applications Procedure Materials Notes Related Products

Principle

Immunoaffinity chromatography is a method for separating target antibodies or antigens from a heterogeneous solution. It is column-based, which means that the solution is flowed through a column and eluted at the other end. The column is pre-functionalized with the capture antibody or antigen. The target protein is adsorbed onto the resin-bound capture protein and is retained in the column while the remaining solution is eluted. The fraction containing the target protein is later eluted and purified.

Applications

Food Analysis; Biochemisry

Procedure

1. Preparation of Column: The column is loaded with solid support such as sepharose, agarose, cellulose etc.. Ligand is selected according to the desired isolate. Spacer arm is attached between the ligand and solid support.
2. Loading of Sample: Solution containing a mixture of substances is poured into the elution column and allowed to run at a controlled rate.
3. Elution of Ligand-Molecule Complex: Target substance is recovered by changing conditions to favor elution of the bound molecules.

Materials

• Sample: Plants; Natural Food; Protein; Drug; Pollutants; Blood; Saliva; Serum; Plasma; Antibodies; Viruses & More
• Equipment: Agarose; Silica gel; Aluminium oxide; Acrylate; Organic polymers; Wash Buffer
• (Optional): Ligand; Spacer arm; Column

Notes

1. Immunoaffinity chromatography is one of the most widely used affinity-derived procedures, and it permits the production of ligands when the needed ligand is unavailable.
2. In this method, the stationary phase consists of an antibody or an agent linked to antibodies. Due to the high specificity of antibodies, it is possible to isolate varied substances using this method.

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