MagINDEX™ rProtein A/G Magnetic Agarose, Settled Resin, 10 mL, 11-0273-010, Marvelgent biosciences (CAT#: STEM-C-3704-LGZ)

MagINDEX™ rProtein A/G Magnetic Sepharose is an affinity chromatography medium designed for fast, simple, one-step purification of a broad range of immunoglobulins, as well as fusion proteins containing Fc regions.

Proprietary MagIndex™ technology facilitates encapsulation of superparamagnetic cores in a mechanically stable agarose matrix, which is optimal absorbance for high-yield protein purification with minimal background.

Superparamagnetic cores have a very fast magnetic response in a magnetic field, but have no memory when the magnetic force is removed. The agarose-based encapsulation provides a strong shield that protects the core well in a wide range of reagents commonly used in protein purification. In addition, as an ideal protein purification matrix, agarose matrix can achieve high-purity and high-capacity purification of antibodies and Ig fragments in high-throughput screening and various preparation scales.

Superparamagnetic core with fast response to magnetic field
Low non-specific binding, detection accuracy
High protein A/G ligand density for optimal yield of antibody and Ig fragments
High stability in common buffer systems and detergents

Protein A is a bacterial cell wall protein isolated from Staphylococcus aureus that binds to the Fc region of most immunoglobulins through interaction with the heavy chain. Native protein A contains five high-affinity IgG-binding domains and many domains with unknown functions. Native protein G has three IgG binding domains as well as albumin and cell surface binding sites, while recombinant protein G has only an Ig recognition domain and no other non-specific sequences.

Recombinant protein A/G is a recombinant chimeric protein consisting of the IgG binding domains of protein A and protein G. Protein A/G contains 5 binding domains of protein A and 2 binding domains of protein G. Protein A/G binding is less pH-dependent than protein A, but otherwise has the additivity of protein A and protein G. Therefore, Protein A/G is an ideal protein to bind the broadest IgG subclass in rabbit, mouse, human, and other mammalian samples.

Size: 10 mL
Ligand: Recombinant chimeric protein A/G containing only Ig binding domains
Binding capacity: >10 mg human IgG/mL medium
Matrix: Cross-linked agarose beaded particles containing the superparamagnetic core, supplied as a 20% slurry
Particle size: 30 μm - 100 μm
pH stability: Long term: pH 2 - 9; Short term: pH 2 - 11
Storage temperature: 2 - 8°C
Storage buffer: PBS containing 0.01% Tween-20 and 0.02% NaN3

High capacity, > 10 mg human IgG / mL of settled resins
High selectivity for high purity

Chromatography Columns, 1 Adjustable End, 5-7bar, 10mm ID, 800mm Length, 7bar, GB-10-800-1TE, G-BIOSCIENCES (CAT#-STEM-C-3389-LGZ)
Chromatography Columns, 1 Adjustable End, 5-7bar, 10mm ID, 900mm Length, 7bar, GB-10-900-1TE, G-BIOSCIENCES (CAT#-STEM-C-3390-LGZ)
Chromatography Columns, 1 Adjustable End, 5-7bar, 10mm ID, 1000mm Length, 7bar, GB-10-1000-1TE, G-BIOSCIENCES (CAT#-STEM-C-3391-LGZ)
Chromatography Columns, 1 Adjustable End, 5-7bar, 15mm ID, 100mm Length, 7bar, GB-15-100-1TE, G-BIOSCIENCES (CAT#-STEM-C-3392-LGZ)
Chromatography Columns, 1 Adjustable End, 5-7bar, 15mm ID, 200mm Length, 7bar, GB-15-200-1TE, G-BIOSCIENCES (CAT#-STEM-C-3393-LGZ)
Chromatography Columns, 1 Adjustable End, 5-7bar, 15mm ID, 300mm Length, 7bar, GB-15-300-1TE, G-BIOSCIENCES (CAT#-STEM-C-3394-LGZ)