Malondialdehyde Determination in Plant Tissues

When plants are exposed to abiotic stress, they will simulate the formation of reactive oxygen species (ROS) whose damaging effect include peroxidation of membrane lipid. Malondialdehyde (MDA) is the end product of membrane lipid peroxidation in plant tissues under adversity conditions. The content of malondialdehyde in plant tissues indicate the rate and intensity of cell membrane lipid peroxidation and is closely related to plant aging. Malondialdehyde is cytotoxic and can lead to cross-linked polymerization of proteins, nucleic acids, and formation of various adducts that damage biomolecules. Malondialdehyde is widely used as a reliable marker to determine the degree of plant injury caused by environmental stress. The more the plant is damaged, the higher its MDA content.

STEMart is able to efficiently and accurately determine the content of MDA in plant tissues to evaluate the strength of plant response to adverse conditions.

Test Samples

Plant tissue

Test Methods

• Thiobarbituric acid (TBA) assay. This assay relies on the formation of red-colored MDA-(TBA)2 adduct during the chromogenic reaction of TBA with MDA under acidic conditions (e.g., glacial acetic acid or sulfuric acid) and elevated temperatures (e.g., 95 °C). the absorbance values of MDA-(TBA)2 adduct at 532nm wavelength are compared to calculate the MDA content.
• Dinitrophenyl hydrazine (DNPH) assay. This assay relies on reaction of derivatization reagents 2, 4- dinitrophenyl hydrazine (DNPH) with the carbonyl groups of MDA. The reaction product can be quantified by HPLC, GC-MS, or LC-MS/MS methods.
• Pentafluorobenzyl (PFB) bromide assay. The PFB bromide can alkylate MDA at its central C atom, which causes both carbonylic groups to intact. The MDA-(PFB)2 derivative can be quantified by GC-MS or GC-MS/MS analysis.

Why Choose Us

• Comprehensive test report including relevant experimental steps, relevant parameters of the experiment, original pictures, original data, and complete experiment results
• Rich experience in MDA determination
• Customized service for plant research
• Professional instruments and equipment for reliable data and results
• Rapid turnaround and cost-effective

For more information about our plant MDA determination service, please contact us.