Measurement of conformational changes in serum albumin by SDSL EPR (CAT#: STEM-MB-1037-WXH)

Introduction

Serum albumin, often referred to simply as blood albumin, is an albumin (a type of globular protein) found in vertebrate blood. Human serum albumin is encoded by the ALB gene. Other mammalian forms, such as bovine serum albumin, are chemically similar.
Serum albumin is produced by the liver, occurs dissolved in blood plasma and is the most abundant blood protein in mammals. Albumin is essential for maintaining the oncotic pressure needed for proper distribution of body fluids between blood vessels and body tissues; without albumin, the high pressure in the blood vessels would force more fluids out into the tissues. It also acts as a plasma carrier by non-specifically binding several hydrophobic steroid hormones and as a transport protein for hemin and fatty acids.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Electron Paramagnetic Resonance (EPR), also called Electron Spin Resonance (ESR), is a branch of magnetic resonance spectroscopy which utilizes microwave radiation to probe species with unpaired electrons, such as radicals, radical cations, and triplets in the presence of an externally applied static magnetic field.
EPR spectroscopy is particularly suitable for the investigation of (bio)chemical systems with strongly localized spin density and their interaction with the environment. For these systems EPR provides information on the structure and dynamics and is widely used in chemistry, physics and biology.
Site-directed spin labeling (SDSL) is a technique for investigating the structure and local dynamics of proteins using electron spin resonance. The theory of SDSL is based on the specific reaction of spin labels with amino acids. A spin label's built-in protein structure can be detected by EPR spectroscopy. SDSL is also a useful tool in examinations of the protein folding process.

Applications

• Study dynamic organisation of lipids in biological membranes, lipid-protein interactions and temperature of transition of gel to liquid crystalline phases.
• Determine oxygen levels in tissues and blood.
• Injection of spin-labeled molecules allows for electron resonance imaging of living organisms.
• EPR can be used to measure microviscosity and micropolarity within drug delivery systems as well as the characterization of colloidal drug carriers.
• The study of radiation-induced free radicals in biological substances (for cancer research).
• Investigation on the antioxidant properties of medicine

Procedure

1. Sample Preparation
2. Electron paramagnetic resonance (EPR) spectroscopy testing
3. Data analysis

Materials

• EPR Spectrometer
• Spectrophotometer

Other recommended products

Investigations on the HIV-1 reverse transcriptase by SDSL EPR (CAT#: STEM-MB-1034-WXH)
Monitoring of tissue oxygenation in the brain by Electron paramagnetic resonance (EPR) spectroscopy (CAT#: STEM-MB-1009-WXH)
Analysis of Amyloid β (Aβ) by SDSL EPR (CAT#: STEM-MB-1024-WXH)
Analysis of synaptosomal membrane-protein by Electron paramagnetic resonance (EPR) spectroscopy (CAT#: STEM-MB-1089-WXH)
Analysis of phospholipid bilayers by Electron paramagnetic resonance (EPR) spectroscopy (CAT#: STEM-MB-1093-WXH)
Analysis of Charge and potential of cells/membranes by Electron paramagnetic resonance (EPR) spectroscopy (CAT#: STEM-MB-1041-WXH)
Analysis of cellular viscosity by Electron paramagnetic resonance (EPR) spectroscopy (CAT#: STEM-MB-1042-WXH)
Investigation on the effects of calcium channel blocker by Electron paramagnetic resonance (EPR) spectroscopy (CAT#: STEM-MB-1019-WXH)