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Micro- and nano-scale plastic particles in the environment result from their direct release and degradation of larger plastic debris. Relative to macro-sized plastics, these small particles are of special concern due to their potential impact on marine, freshwater, and terrestrial systems. While microplastic (MP) pollution has been widely studied in geographic regions globally, many questions remain about its origins. It is assumed that urban environments are the main contributors but systematic studies are lacking. The absence of standard methods to characterize and quantify MPs and smaller particles in environmental and biological matrices has hindered progress in understanding their geographic origins and sources, distribution, and impact. Hence, the development and standardization of methods is needed to establish the potential environmental and human health risks. In this service, we used stable carbon isotope ratio mass spectrometry (IRMS) as complementary techniques for characterization of common plastics.