Monitoring of BHK-21 Cell Metabolism and Growth by UV-Vis Spectroscopy (CAT#: STEM-MB-0901-WXH)

Introduction

Among mammalian cells, the BHK-21 cell line has found applications in veterinarian viral vaccines and heterologous protein production, even in the transient mode. Many media formulations for mammalian cell culture often contain an optional pH indicator, phenol red. This dye provides a useful, visual but qualitative mode for monitoring metabolism within a culture device. The aim of this work was to calibrate a Partial Least Squares (PLS) model for glutamine, glutamate, glucose, lactate, and viable cell concentration based on off-line ultraviolet-visible spectra for BHK-21 suspension cell culture in bioreactors.

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Principle Applications Procedure Materials Notes Related Products

Principle

UV-Vis spectroscopy is an analytical technique that measures the amount of discrete wavelengths of UV or visible light that are absorbed by or transmitted through a sample in comparison to a reference or blank sample. This property is influenced by the sample composition, potentially providing information on what is in the sample and at what concentration. The only requirement is that the sample absorb in the UV-Vis region, i.e. be a chromophore. Absorption spectroscopy is complementary to fluorescence spectroscopy. Parameters of interest, besides the wavelength of measurement, are absorbance (A) or transmittance (%T) or reflectance (%R), and its change with time.

Applications

UV/Vis spectroscopy is routinely used in analytical chemistry for the quantitative determination of diverse analytes or sample, such as transition metal ions, highly conjugated organic compounds, and biological macromolecules. Spectroscopic analysis is commonly carried out in solutions but solids and gases may also be studied.

Procedure

1. Calibrate the Spectrometer
2. Perform an Absorbance Spectrum
3. Kinetics Experiments with UV-Vis Spectroscopy

Materials

UV/VIS Spectrophotometer

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