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Monodehydroascorbate Reductase Analysis in Plant Tissues

All kinds of abiotic and biotic stresses significantly limit plant productivity by disturbing cellular biochemistry and physiology via the overproduction of activated oxygen molecule-reactive oxygen species (ROS). Uncontrolled accumulation of ROS results in oxidation of DNA and RNA, protein denaturation and decreased enzyme activity as well as lipid peroxidation and carbohydrate oxidation. Within the antioxidant system in plants, the ascorbate-glutathione (AsA-GSH) path-way plays an important role in regulating and controlling the content of ROS.

Monodehydroascorbate reductase (MDAR) is located in the chloroplasts, cytosol, mitochondria and peroxisomes. As a regulatory enzyme of the AsA-GSH path-way, MDAR plays a critical role in maintaining the optimal concentration of ascorbate by catalyzing the reduction of MDA radical at the expense of NAD(P)H oxidation. The activity of MDAR increases under several stress conditions, such as drought, salinity, high light intensity, and low temperature.

STEMart provides comprehensive analysis services to identify and investigate the function of MDAR in regulating plant environmental stress responses.

Test Samples

Plant tissue

Test Capabilities

  • Identification of MDAR genes
  • Phylogenetic analysis of MDAR genes
  • Structure analysis of MDAR genes
  • Expression profile of MDAR genes of plant under stress conditions
  • 3D structure and physicochemical properties of MDAR genes
  • Subcellular localization of MDAR enzymes and Isoenzymes analysis
  • Activity determination of MDAR

Why Choose Us

  • Comprehensive test report including relevant experimental steps, relevant parameters of the experiment, original pictures, original data, and complete experiment results
  • Customized service for plant research
  • Professional instruments and equipment for reliable data and results
  • Rapid turnaround and cost-effective

For more information about our plant MDAR analysis service, please contact us.

Reference

  1. Foyer C.H., Noctor G. (2011). “Ascorbate and glutathione: the heart of the redox hub”. Plant Physiol. 155(1): 2–18.

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