NHS-Activated Agarose (Dry Form) With Spin Columns, 5 g With 5x 3 ml Spin Columns, 786-1214, G-BIOSCIENCES (CAT#: STEM-C-3105-LGZ)

NHS-activated agarose consists of 6% cross-linked agarose activated by the addition of reactive NHS (n-hydroxysuccinimide) groups. NHS groups form covalent, chemically stable amide bonds with primary amine-containing ligands. NHS-activated agarose also contains a spacer between the NHS group and the agarose beads, making it suitable for conjugation of small proteins and peptides. The long spacer arm minimizes steric hindrance, allowing efficient binding of ligands, including small proteins and peptides.

6% highly cross-linked agarose beads coupled to a 6-carbon spacer. The terminal carboxyl group of the spacer arm is activated by esterification with the NHS group.

The coupling reaction is carried out in an amine-free buffer at pH 7-9, and the coupling efficiency is typically 80% regardless of the pI or molecular weight of the ligand. Once the ligand is coupled to the resin, the resin can be used for multiple affinity purifications. This resin is suitable for gravity flow and low to medium pressure applications.

Size: 5 g with 5x 3 ml Spin Columns

High level of activation
Simple: one-step reaction
Stable: The resulting affinity chromatography is very stable, especially at pH extremes
Spacer arm between agarose and reactive group to minimize steric hindrance
Suitable for small proteins and peptides
Fast: 80% coupling in the first 30 minutes
Compatible with any ligand and primary amine
Generate reusable affinity columns

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