Sephadex G-10, 500 g, Cytiva (CAT#: STEM-C-1587-LC)

Sephadex G-10 manufactured by Cytiva is a well established gel filtration resin for desalting and buffer exchange of peptides and small biomolecules>700 molecular weight. Sephadex G-10 is prepared by crosslinking dextran with epichlorohydrin. Sephadex G-10 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules.

Matrix: Cross-linked dextran
Particle size distribution, wet: 55-165 µm
Particle Size Dry: 40 µm-120 µm
pH stability, operational1: 2-13
Flow rate2: < Darcy´s Law
Pressure/Flow Specification: 24-49 cm/h, pressure drop cm H2O/bed height = 2, bed height 30 cm, 2.6 cm i.d
Fractionation range (Mr) (globular proteins): <7 × 10²
Fractionation (Mp) Dextrans: << 7 x 10²
Exclusion Limit:> 700
Storage: 4 to 30°C
Autoclavable: In wet form (pH 7.0) at 120°C for 30 min
Pack size: 500 g

Smallest exclusion limit in the Sephadex range

Superdex 75 Prep Grade, 1 L, Cytiva (CAT#: STEM-C-1639-LC)