NW Rose Series Histidine-Tagged Protein Purification Resins, NW Rose Ni FF 10L (CAT#: STEM-C-3742-ZJF)

Immobilized metal affinity chromatography (IMAC) is a highly effective technique for purifying recombinant proteins with a histidine-rich tag (His-tag) at the N- or C-terminus, as well as proteins naturally abundant in histidine or cysteine residues. This method enables efficient capture of target proteins directly from raw cell culture harvests, followed by selective elution using appropriate concentrations of imidazole. This process effectively separates the target proteins from host cell impurities, such as proteins and nucleic acids.
A range of IMAC resins is available, either pre-charged with Ni²⁺ or uncharged, designed for the preparation of highly pure recombinant proteins. These resins are suitable for applications in scientific research, diagnostic assays, and therapeutic development.

Chromatography Technique: Histidine-tagged protein purification
Matrix: Cross-linked agarose with fast flow rate
Particle Size: 45-165 μm
Ligand: NTA
Dynamic Binding Capacity: ~40 mg·mL⁻¹
Metal Ion Capacity: 15 μmol·mL⁻¹ (Ni²⁺)
Maximum Pressure: 0.3 MPa
Clean In Place: 0.5 M NaOH (Ni²⁺ must be removed)
Maximum Flow Velocity: 150 cm/h (50/30 Column, bed height 10 cm)
Recommended Flow Velocity: 600 cm/h (16/20 Column, bed height 10 cm)
pH Stability: 3-12 (operational), 2-14 (CIP, Ni²⁺ must be removed)
Chemical Stability: Stable in commonly used aqueous buffers, 0.1 M HCl, 0.1 M NaOH, 2% SDS, 30% isopropyl alcohol, 6 M guanidine hydrochloride, 8 M urea, 70% ethanol (if Ni²⁺ is removed)
Operational Temperature: 4-30 ℃
Storage: 20% ethanol, 4-30 ℃

Outstanding Pressure-Flow Properties: Polymeric or cross-linked agarose particles with excellent pressure-flow characteristics.
Efficient Mass Transfer & High Binding Capacity: Small elution volumes and high binding capacity for effective purification.
Minimal Non-Specific Binding: Ensures excellent recovery of His-tagged proteins with minimal interference.
Low Metal Leaching & Excellent Chemical Compatibility: Ensures high performance with low metal leaching and broad chemical compatibility.
Uncharged Resins for Metal Immobilization: Uncharged resins available for immobilizing divalent metals (Zn2+, Cu2+, Co2+, Fe3+) for selective protein binding.

STEM-C-3740-ZJF
STEM-C-3741-ZJF
STEM-C-3743-ZJF
STEM-C-3744-ZJF