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Plant Phosphorus Elements Determination

Phosphorus is a nonmetallic chemical element which is usually absorbed by plants from soil in the form of H2PO4− and HPO42−. When phosphorus enters the plant, most of it becomes organophosphorus compounds (phosphate monoester (C-O-P bond) (e.g., phytate), phosphate diesters (C-O–P–O-C bond) (e.g., nucleotides and phospholipids), α- and β-glycerophosphates, and organic polyphosphates), and some of it remains in the form of inorganic (orthophosphate, pyrophosphate (P-O-P bond), and polyphosphate). The phosphorus concentration in plants ranges from 0.05 to 0.5% of plant dry weight.

Fig.1 6-1-3-2-plant-phosphorus-elements-determination-1 Fig. 1 The occurrence and translocation of various forms of phosphorus in soil and plant

Phosphorus is an essential nutrient element which makes plants grow and develop well and improves plant resistance. It can promote all kinds of metabolism and plays a crucial role in plant ATP response, sugar metabolism, protein metabolism, and fat metabolism, so the determination of phosphorus content in plants must be analyzed in plant research.

STEMart is able to efficiently and accurately determine the content of phosphorus elements in plant samples.

Test Samples

Plant tissue samples

Test Capabilities

  • Determination of total phosphorus (TP) in plant samples
  • Determination of inorganic phosphorus in plant samples
  • Determination of organic phosphorus in plant samples

Test Methods

  • Ion chromatography (IC)
  • Spectrophotometric methods
    • Molybdenum blue colorimetric method
    • Malachite green assay
  • Spectrometric methods
    • FAAS
    • ETAAS
    • ICP-OES
    • ICP-AES
    • ICP-MS
  • NMR spectroscopy

For more information about our plant phosphorus elements determination service, please contact us.

References

  1. McKie V.A., McCleary B.V. (2016). “A novel and rapid colorimetric method for measuring total phosphorus and phytic acid in foods and animal feeds”. J AOAC Int. 99:738–43.
  2. Zhu J, Loubéry S, Broger L, Zhang Y, Lorenzo-Orts L, Utz-Pugin A, Fernie AR, Young-Tae C, Hothorn M. (2020). “A genetically validated approach for detecting inorganic polyphosphates in plants”. Plant J. 102:507–16.
  3. Telgheder, U., Malinowski, M., & Jochmann, M. A. (2009). “Determination of volatile organic compounds by solid-phase microextraction—gas chromatography-differential mobility spectrometry”. International Journal for Ion Mobility Spectrometry. 12(4), 123-130.

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