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Profiling of regioisomeric triacylglycerols in edible oils by supercritical fluid chromatography/tandem mass spectrometry (CAT#: STEM-CT-0060-LJX)

Introduction

Triacylglycerol is an organic compound formed by esterification of three hydroxyl groups of glycerol with three fatty acid molecules. It is a non-polar substance that is stored in the body in a non-hydrated form and is the energy substance with the largest reserves and the most productive capacity in the body. Plant triacylglycerol is mostly oil, animal triacylglycerol is mostly fat. Solid and liquid triacylglycerols are collectively referred to as fats.
Supercritical fluid chromatography (SFC) coupled with triple quadrupole mass spectrometry was applied to the profiling of several regioisomeric triacylglycerols (TAGs).




Principle

Supercritical fluid is a substance that has both gaseous and liquid properties above the critical point. Supercritical fluids have the advantages of high diffusion coefficient, low viscosity, adjustable solubility and high vapor phase density, so they can provide efficient mass spectrometry ionization and separation results.
Supercritical fluid chromatography (SFC) is an efficient separation technique that uses supercritical fluid as a mobile phase. The samples are packed into short tubes or SPE columns, and the samples are compressed and regulated by supercritical fluid to obtain good solubility. The sample components are then separated by column interaction, thus achieving the separation of different compounds.

Applications

For efficient separation of substances
Widely used in biology, chemistry, environmental protection and other fields

Procedure

1. Sample injection
2. The high pressure pump increases the pressure of the sample and mobile phase
3. The sample and mobile phase enter the chromatographic column
4. Flow limiter assists sample separation

Materials

• Sample Type:
Edible oils

Notes

1. In the process of separation, the control of pressure and temperature of supercritical fluid is very important, which affects the properties and separation efficiency of supercritical fluid.
2. In addition, the selection of the appropriate column, packing and moving equivalent factors will also affect the separation effect.
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