PROTEINDEX™ Anti-DYKDDDDK Agarose 4 Fast Flow, 10 mL, 18-0270-010, Marvelgent biosciences (CAT#: STEM-C-3733-LGZ)

PROTEINDEX™ Anti-DYKDDDDK Sepharose 4 Fast Flow is an affinity chromatography medium for the purification of proteins containing the peptide sequence DYKDDDDK (n-asp-tir-lys-asp-asp-asp-lys-c). The ligand covalently immobilized on the matrix support is a recombinant mouse monoclonal IgG1 species that binds to the same epitope as Sigma's Anti-FLAG® M2 antibody and which is highly sensitive to the peptide DYKDDDDK in a calcium-independent manner specificity. Commonly referred to as the FLAG® epitope, the amino acid sequence DYKDDDDK is used as an affinity tag, fused at the N-terminus, C-terminus, or an internal position of the recombinant protein.

For protein purification, ProteIndex™ Anti-DYKDDDDK Sepharose 4FF Resin is added to the sample. After capturing dykddddk-tagged proteins, unbound and non-specifically bound proteins can be washed away. The bound target protein can be eluted with a competing peptide under mild conditions or with an acidic glycine-HCl buffer.

Since the binding between the epitope and the antibody can occur under natural conditions, any proteins or other macromolecules capable of interacting with the tagged fusion protein will also be captured during the binding step. Therefore, anti-dykddddk 4FF affinity resin can be used for co-immunoprecipitation to analyze protein complexes associated with fusion proteins of interest.

The base matrix of ProteIndex™ Anti-DYKDDDDK Agarose 4FF is highly cross-linked 4% agarose, which provides strong support for low background and high physical stability for chromatography procedures of all scales. Affinity resins are recommended for gravity/batch applications, spin cleanup columns, or cartridges for FPLC instruments.

Size: 10 mL
Ligand: Mouse monoclonal anti-DYKDDDDK IgG1, calcium-independent
Binding capacity: ≥ 3 mg DYKDDDDK-tagged protein (~ 50 kDa) per mL medium
Purity: ≥ 90%
Matrix: 4% highly cross-linked agarose supplied as 50% slurry
Particle size: 45 μm - 165 μm with the average particle size being 90 μm
Maximum pressure: 0.3 MPa (3 bar)
Recommended flow rate: 30 - 250 cm/hour
pH stability: 3-12
Storage temperature: 2 - 8°C
Storage buffer: 1x PBS with 0.02% NaN3

High selectivity for high purity (>90%)
> 3 mg DYKDDDDK-tagged protein (50 kDa)/mL medium
Ligand coupling is stable and leakage is low; it can be reused for the purification of the same protein (it can be reused 3~5 times during acid elution)
Versatile - ideal for gravity/batch, spin column and FPLC applications

Chromatography Column, Fixed Volume, 5-7bar, 15mm ID, 300mm length, 7bar, GB-15-300, G-BIOSCIENCES (CAT#-STEM-C-3594-LGZ)
Chromatography Column, Fixed Volume, 5-7bar, 15mm ID, 400mm length, 7bar, GB-15-400, G-BIOSCIENCES (CAT#-STEM-C-3595-LGZ)
Chromatography Column, Fixed Volume, 5-7bar, 15mm ID, 500mm length, 7bar, GB-15-500, G-BIOSCIENCES (CAT#-STEM-C-3596-LGZ)
Chromatography Column, Fixed Volume, 5-7bar, 15mm ID, 600mm length, 7bar, GB-15-600, G-BIOSCIENCES (CAT#-STEM-C-3597-LGZ)
Chromatography Column, Fixed Volume, 5-7bar, 15mm ID, 700mm length, 7bar, GB-15-700, G-BIOSCIENCES (CAT#-STEM-C-3598-LGZ)
Chromatography Column, Fixed Volume, 5-7bar, 15mm ID, 800mm length, 7bar, GB-15-800, G-BIOSCIENCES (CAT#-STEM-C-3599-LGZ)