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ProteoSure™ Recombinant Carboxypeptidase B, 100 mg x10, 14-RCPB-1G, Marvelgent biosciences (CAT#: STEM-GT-3040-LGZ)

Highlights

100% Free of Animal Origin
By using Marvelgent's recombinant carboxypeptidase B, it helps avoid contamination by viruses and any other potential adventitious agents, which can be found in animal-derived carboxypeptidase B.

Cat Number: STEM-GT-3040-LGZ

Application: It is used to catalyze the hydrolysis of lysine, arginine and histidine at the C-terminus.

Model: 14-RCPB-1G

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Description

Carboxypeptidase B catalyzes the hydrolysis of lysine, arginine, and histidine at the C-terminus of polypeptides. Its molecular weight is 33.8 kDa, the optimum pH is 8.0, and the pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. This enzyme is also inhibited by metal chelators such as EDTA.

Marvelgent's recombinant carboxypeptidase B was expressed in E. coli and purified by high pressure liquid chromatography. There is no trace contamination of proteases, such as carboxypeptidase A or chymotrypsin, and no protease inhibitors, such as PMSF.

Specification

Size: 100 mg x10
CAS #: 9025-24-5
Enzyme Commission #: 3.4.17.2
Molecular weight: 34.5 kDa
Origin of species: Rat
Source: E.coli
Purified by: HPLC
Physical form: White to off-white lyophilized powder
Additives: Tris salts, NaCl and carbohydrates.
Protein content: 35%~60%
Specific activity: ≥170 Units/mg protein
Unit definition: One unit of carboxypeptidase B activity hydrolyzes one micromole of hippuryl-L-arginine per minute at 25℃, pH 7.65.
Purity: > 95%
Contaminant activity: No chymotrypsin, carboxypeptidase A, or other protease contaminants. Less than 10 ppm of recombinant trypsin.
Storage: Store in a sealed container at 2 - 8°C. Once reconstituted, it should be stored at -20°C. The product is stable for 24 months. Greater than 90% enzymatic activity retains after 10 repeated freeze-and-thaw cycles.
Intended use: For research or further processing only. Not intended for any human or animal diagnostic or therapeutic use.

Features

100% Free of Animal Origin
By using Marvelgent's recombinant carboxypeptidase B, it helps avoid contamination by viruses and any other potential adventitious agents, which can be found in animal-derived carboxypeptidase B.

High purity
Recombinant carboxypeptidase B exhibits highly specific enzymatic activity without contaminating proteases, such as chymotrypsin or carboxypeptidase A, commonly found in enzymes of animal origin.

Stablize
Sterile freeze-dried powder is provided with high stability in transportation and storage.

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