Recombinant Human CTLA-4 Protein, Seafrom (CAT#: STEM-CT-0153-ZJF)

The Recombinant Human CTLA-4 Protein manufactured by Seafrom is suitable for immune checkpoint regulation.
CTLA-4, also known as cytotoxic T-lymphocyte-associated protein 4, or CD152 (cluster of differentiation 152), is a protein receptor that plays a role in downregulating the immune system. It is a member of the immunoglobulin superfamily and is expressed on the surface of helper T cells, where it transmits inhibitory signals to T cells. The protein consists of an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. There are alternate splice variants that encode different isoforms.
CTLA-4 is structurally similar to the co-stimulatory protein CD28, and both molecules bind to CD80 and CD86 (also known as B7-1 and B7-2) on antigen-presenting cells. While CD28 transmits stimulatory signals, CTLA-4 delivers inhibitory signals to T cells. CTLA-4 is also present intracellularly in regulatory T cells, where it may play a crucial role in their function. The expression of CTLA-4 increases in T cells following activation through the T cell receptor and CD28. CTLA-4 acts as an inhibitory receptor for B7 molecules.
Clinical trials have explored the use of a fusion protein of CTLA-4 and antibodies (CTLA4-Ig) for rheumatoid arthritis treatment.
The freeze-dried (lyophilized) form of CTLA-4 can be stored at 4℃ for up to 24 months. Once reconstituted, the liquid can be stored at -20℃ for 6-12 months, with precautions taken to avoid repeated freeze-thaw cycles.

Expression Host: Human HEK293 cells
Source: Human
Synonyms: CTLA4, CD152
Protein Sequence: The DNA sequence encodes human CTLA-4 (NP_005205.2), expressed with a His tag at the C-terminus.
Molecular Weight: Recombinant human CTLA-4 protein contains 125 amino acids, with a predicted theoretical molecular weight of 15.3 kD.
Purity: ≥95%, confirmed by SDS-PAGE gel and high-performance liquid chromatography (HPLC) analysis.
Endotoxin: <0.1 EU/μg
Biological Activity: Immobilized B7-2 protein at a concentration of 5 μg/mL (100 μL/well) can bind to CTLA-4 protein in a linear range of 0.5-10 ng/mL. Immobilized B7-1 protein at a concentration of 5 μg/mL (100 μL/well) can bind to CTLA-4 protein in a linear range of 0.1-8 ng/mL.
Purification Method: Chromatographic purification
Composition: Sterile PBS (pH 7.4) freeze-dried powder containing 6% mannitol.

Optimal Stability
Gmp-Grade Quality
Low Batch-to-Batch Variability
Exceptional Specificity
Robust Bioactivity
Efficient and Reliable Performance

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