Recombinant Human SIRPα Protein, Seafrom (CAT#: STEM-CT-0118-ZJF)

The Recombinant Human SIRPα Protein manufactured by Seafrom is suitable for immune cell activation and phagocytosis.
Tyrosine-protein phosphatase non-receptor type substrate 1 (SHPS1), also known as CD172 antigen-like family member A (CD172a), Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha (SIRPA or SIRP alpha), or p84, is a member of the SIRP (signal-regulatory protein) family and belongs to the immunoglobulin superfamily.
SIRP alpha is a cell surface receptor that interacts with CD47 and acts as a docking protein, inducing the translocation of PTPN6, PTPN11, and other binding partners from the cytosol to the plasma membrane. SIRPA or SHPS-1 plays a role in supporting adhesion of cerebellar neurons, promoting neurite outgrowth, facilitating glial cell attachment, and is involved in intracellular signaling during synaptogenesis and synaptic function. Additionally, SIRPA or MyD1 is implicated in the negative regulation of cellular responses initiated by receptor tyrosine kinase activation through cell adhesion, growth factors, or insulin. It also mediates the negative regulation of phagocytosis, mast cell activation, and dendritic cell activation. CD47 binding to SIRPA prevents the maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
Storage Information:
Lyophilized samples can be stored at 4°C for 24 months, and once reconstituted, the liquid can be stored at -20°C for 6-12 months, with care taken to avoid repeated freeze-thaw cycles.

Expression Host: Human HEK293 cells
Source: Human
Synonyms: SHPS1, SIRPA, CD172A, BIT, MFR, MYD1, P84, PTPNS1
Protein Sequence: The DNA sequence encodes human SIRPα (NCBI Reference Sequence: NP_542970.1), expressed with a His tag at the C-terminus.
Molecular Weight: Recombinant human SIRPα protein contains 241 amino acids, with a predicted theoretical molecular weight of 39 kD.
Purity: ≥95%, confirmed by SDS-PAGE gel and high-performance liquid chromatography (HPLC) analysis.
Endotoxin: <0.1 EU/μg
Biological Activity: The ability to bind to human CD47 (Fc tag) is confirmed at 5 μg/mL (100 μL/well), with a linear range of 10-500 ng/mL.
Purification Method: Chromatographic purification
Composition: Sterile PBS (pH 7.4) freeze-dried powder containing 6% mannitol.

Optimal Stability
Gmp-Grade Quality
Low Batch-to-Batch Variability
Exceptional Specificity
Robust Bioactivity
Efficient and Reliable Performance

Recombinant Human Galectin-9 Protein, Seafrom
Recombinant Human TIM-3 Protein, Seafrom
Recombinant Human BTLA Protein, Seafrom
Recombinant Human HER2 Protein, Seafrom