Semi-quantitative PCR (CAT#: STEM-MB-0228-WXH)

Introduction

Semi-quantitative PCR, a PCR technique developed from the traditional PCR qualitative analysis method, is capable of determining the relative amount of the target product. The traditional PCR analysis method determines the presence or absence of the target product by amplifying DNA or cDNA as a template. In contrast, semi-quantitative PCR determines the relative amount of target product in a sample by first determining the minimum number of cycles of limited PCR amplification of the target to a maximum and then comparing the number of PCR amplification products of the target between different samples. It is a simple and specific method commonly used for quantitative DNA and RNA detection.<br />Semi-quantitative is a method intermediate between quantitative and qualitative to determine whether a gene is differentially expressed. Gene expression is compared based on the brightness of the electrophoretic bands, controlling for other variables.