Sephadex G-25 Medium, 500 g, Cytiva (CAT#: STEM-C-1602-LC)

Sephadex G-25 manufactured by Cytiva is a well established gel filtration resin for desalting and buffer exchange in industrial applications. Sephadex G-25 is prepared by crosslinking dextran with epichlorohydrin. Sephadex G-25 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-25 is available in 4 different particle sizes (Coarse, Medium, Fine & Superfine).

BioProcess resin: Yes
Matrix: Cross-linked dextran
pH stability, operational1: 2-13
pH stability, CIP2: 2-13
Pressure/Flow Specification:>100 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.
Fractionation range (Mr) (globular proteins): 1 × 10³-5 × 10³
Fractionation (Mp) Dextrans: 1 x 10²-1 x 10²
Exclusion limit (Mr) (globular proteins): ~5 × 10³
Exclusion Limit: Mr 5000
Storage: 4 to 30°C, dry resins. Used resins 4 to 8 °C in 20% Ethanol or 0.01 M NaOH
Chemical stability: Commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 0.1 M acetic acid, 8 M urea, 6 M guanidine HCl, 24 % ethanol
Certificate of Analysis: Yes
Swelling factor: ~1.7
Particle size distribution, dry3: 50 - 150 µm
Pack size: 500 g

Excellent recovery and minimum sample dilution
Meets industrial demands with security of supply and comprehensive technical and regulatory support

Sephacryl S-300 HR, 10 L, Cytiva (CAT#: STEM-C-1625-LC)