Sephadex LH-20, 500 g, Cytiva (CAT#: STEM-C-1583-LC)

Sephadex LH-20 resin manufactured by Cytiva is used for preparative purification of natural products such as steroids, terpenoids, lipids, and low molecular weight peptides in organic solvents. This chromatography resin is made from cross-linked Sephadex G-25 dextran beads, hydroxypropylated to yield both hydrophilic and lipophilic characteristics.

Matrix: Hydroxypropylated, cross-linked dextran
Particle Size: Dry 18 µm-111 µm
Particle Size in Methanol Min.: 27 µm
Particle Size in Methanol Max.: 163 µm
Sample Loading Volume: Determined by desired degree of resolution [Adsorption Mode], ~2% total bed volume [Molecular Sizing], ~1% total bed volume [Partition Mode]
Application: For fractionation of lipids, hormones, vitamins, and other small biomolecules in polar organic solvents and aqueous solvent mixtures
pH stability, operational1: 2-13
pH stability, CIP2: 2-13
Pressure/Flow Specification: 25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d.
Flow velocity: < 60 cm/hour
Fractionation range (Mr) (globular proteins): 4 × 10³ (E×clusion limit will depend on the solvent)-5 × 10³ (E×clusion limit will depend on the solvent)
Exclusion Limit: 4 x 10³ (for Peptides) 5 x 10³ (small organics)
Exclusion Limit Peptides: 4 x 10³
Exclusion Limit Small Organics: 5 x 10³
Storage: 4 to 30°C
Estimated shelf life from manufacture date: 8 years
Chemical stability: Stable in most aqueous and organic eluent systems.
Avoid Using: Not stable below pH 2 nor to strong oxidising agents
Autoclavable: 20 min at 121°C
Pack size: 500 g

Broad stability in most aqueous mixtures and polar organic solvents
Powerful chromatographic selectivity from the matrix's dual hydrophilic/lipophilic nature
High chemical and physical robustness
High reproducibility from batch to batch

Superdex 30 Prep Grade, 150 mL, Cytiva (CAT#: STEM-C-1635-LC)