Sephadex G-50 Coarse, 100 g, Cytiva (CAT#: STEM-C-1605-LC)

Sephadex G-50 manufactured by Cytiva is a well established gel filtration resin for purification of DNA from small molecules. Sephadex G-50 is prepared by crosslinking dextran with epichlorohydrin. Sephadex G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is available in 4 different particle sizes (Coarse, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances.

Matrix: Cross-linked dextran
Particle size distribution, wet: 200-610 µm
Particle Size Dry: 100 µm-300 µm
pH stability, operational1: 2-10
Flow rate: < Darcy´s Law
Pressure/Flow Specification: min 400 cm/h, pressure drop cm H2O/bed height=3, bed height 10 cm, column 5 cm i.d.
Fractionation range (Mr) (globular proteins): 1 × 10³-3 × 10⁴
Fractionation (Mp) Dextrans: 5 x 10²-1 x 10⁴
Exclusion Limit>: 3 x 10⁴
Storage: 4 to 30°C
Autoclavable: In wet form (pH 7.0) at 120°C for 30 min
Pack size: 100 g

Sephacryl S-400 HR, 10 L, Cytiva (CAT#: STEM-C-1628-LC)