Services of Proteins by Polyacrylamide Gel Electrophoresis (PAGE) (CAT#: STEM-ET-0328-ZJF)

Introduction

Polyacrylamide gel electrophoresis (PAGE) based on polyacrylamide gel is usually used for separation and detection of proteins and oligonucleotides. SDS-polyacrylamide gel electrophoresis (SDS-PAGE) eliminates the influence of structure and charge in electrophoresis, and proteins are separated based on the differences in their molecular weight. The starting sample could come from any number of sources such as a patient sample, homogenised tissue or bacterial culture. It is also possible to use PAGE to separate DNA and RNA, but proteins are the most common sample type.
We provide protein-related services based on polyacrylamide gel electrophoresis (PAGE) including but not limited to the following:
• Protein separation
• Concentration analysis of protein
• Purity analysis of protein
• Molecular weight analysis of protein
• Detection and separation of oligonucleotide
• Protein purification and characterization
• Identification of protein isoforms and variants
• Analysis of protein-protein interactions
• Detection and quantification of protein contaminants in food and pharmaceuticals
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Principle Applications Procedure Materials Notes Related Products

Principle

Polyacrylamide gel electrophoresis (PAGE) is an electrophoresis which based on polyacrylamide gel. Polyacrylamide gel is tougher than agarose gel and is relatively inert, thermostable, strong, and transparent, with a uniform pore size. PAGE is a widely used technique for separating and analyzing proteins based on their size and charge. It involves the migration of proteins through a porous gel matrix made of cross-linked polyacrylamide under the influence of an electrical field. Known as one of the most versatile and widely used techniques, PAGE has the ability to separate proteins at high resolution, allowing the detection of minor differences in protein composition.

Applications

Molecular biology, genetics, biomedical, clinical, pharmaceutical, forensic, industrial, and food analysis

Procedure

1. Preparation: Prepare electrophoresis buffer with a certain pH. Prepare a gel solution of appropriate concentration, taking care not to produce bubbles, and use a cast to solidify the solution into a gel.
2. Sample Application: Put the prepared gel with a cast into the electrophoresis tank, and add an appropriate amount of buffer. Pipette the sample into the sample wells.
3. Electrophoresis: Adjust the appropriate distance between the electrodes. Switch on the electrophoresis apparatus and set the voltage and current. Perform electrophoresis for a period of time.
4. Determination: After electrophoresis, stain the gel for visible results. Observe, record and analyze the position of separated bands. Or, utilize an imaging system for analysis.
5. Downstream processing: After separation, an additional method is often applied to the separated bands for further processing. For example, cut the band out of the gel as a slice to dissolve and purify it.

Materials

• Gel electrophoresis apparatus
• Sample solution
• Buffer solution and polyacrylamide gel

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