Sizing lipid droplets by Nanoparticle tracking analysis (NTA) (CAT#: STEM-MB-0727-WXH)

Introduction

Lipid droplets, also referred to as lipid bodies, oil bodies or adiposomes, are lipid-rich cellular organelles that regulate the storage and hydrolysis of neutral lipids and are found largely in the adipose tissue. They also serve as a reservoir for cholesterol and acyl-glycerols for membrane formation and maintenance. Lipid droplets are found in all eukaryotic organisms and store a large portion of lipids in mammalian adipocytes.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Nanoparticle tracking analysis (NTA) is a method for visualizing and analyzing particles in liquids that relates the rate of Brownian motion to particle size. The rate of movement is related only to the viscosity and temperature of the liquid; it is not influenced by particle density or refractive index. NTA allows the determination of a size distribution profile of small particles with a diameter of approximately 10-1000 nanometers (nm) in liquid suspension.

Applications

NTA has been used by commercial, academic, and government laboratories working with nanoparticle toxicology, drug delivery, exosomes, microvesicles, bacterial membrane vesicles, and other small biological particles, virology and vaccine production, ecotoxicology, protein aggregation, orthopedic implants, inks and pigments, and nanobubbles.

Procedure

1. Sample preparation
2. Nanoparticle Tracking Analysis
3. NTA Data Processing, Display, and Interpretation

Materials

• Nanoparticle Tracking Analysis (NTA) instrument
• NanoSight instrument
• Nanoparticle Analyzer

Other recommended products

Enumeration and phenotyping of circulating microvesicles by Nanoparticle tracking analysis (NTA) (CAT#: STEM-MB-0719-WXH)
Monitoring microvesicle and exosome secretion from immune cells by Nanoparticle tracking analysis (NTA) (CAT#: STEM-MB-0707-WXH)
Characterization Carbon and Carbon Nanotube by Nanoparticle tracking analysis (NTA) (CAT#: STEM-MB-0737-WXH)
Analysis of solid lipid nanoparticles by Nanoparticle tracking analysis (NTA) (CAT#: STEM-MB-0732-WXH)
Study of Nanotoxicology by Nanoparticle tracking analysis (NTA) (CAT#: STEM-MB-0746-WXH)
Biomaterials Characterization by Nanoparticle tracking analysis (NTA) (CAT#: STEM-MB-0726-WXH)
Investigation of Nanoparticles (NP) intracellular transport by Nanoparticle tracking analysis (NTA) (CAT#: STEM-MB-0740-WXH)
Characterizing protein aggregates by Nanoparticle tracking analysis (NTA) (CAT#: STEM-MB-0711-WXH)