SP6 RNA Polymerase, 25000U, Seafrom (CAT#: STEM-CT-0096-ZJF)

The SP6 RNA Polymerase, 25000U manufactured by Seafrom is suitable Large-scale SP6 RNA polymerase for high-throughput in vitro transcription, facilitating the generation of large amounts of RNA for research.
SP6 RNA Polymerase is a DNA-dependent RNA polymerase that specifically recognizes the SP6 promoter sequence (5'-ATTTAGGTGACACTATAGAAGNG-3'). SP6 RNA Polymerase catalyzes the incorporation of NTPs into single-stranded or double-stranded DNA templates downstream of the SP6 promoter, resulting in the synthesis of RNA complementary to the downstream DNA template. Linear plasmids containing the SP6 promoter or PCR products with the SP6 promoter can both serve as templates for in vitro RNA synthesis.

Source: Expressed in Escherichia coli, expressing the gene for bacteriophage SP6 RNA polymerase
Storage Temperature & Shelf Life: Stored at -20°C, shelf life of 24 months
Enzyme Storage Buffer:
50mM Tris-HCl (pH 7.9), 100mM NaCl, 1mM EDTA, 20mM β-mercaptoethanol, 0.1% Triton® X-100, 50% glycerol
1×RNA Polymerase Reaction Buffer Composition:
40mM Tris-HCl (pH 7.9), 10mM DTT, 6mM MgCl2, 2mM spermidine
Enzyme Activity Unit Definition:
In a 100μL system, 1 unit of enzyme is defined as the amount needed to incorporate 5 nmol of ATP into a polymeric nucleotide at 37°C within one hour
Enzyme Activity Test Conditions:
In a 50μL reaction system containing 1×RNA Polymerase Reaction Buffer, 2mM NTPs (0.5mM each of ATP, UTP, CTP, GTP), 10mM DTT, and 1μg of SP6 promoter-bearing DNA template
Quality Assurance:
SP6 RNA Polymerase does not contain other nucleases such as T7 or T3 RNA polymerase, DNAases, and RNAases.
Specification: 25,000U
Includes:
5× RNA Polymerase Reaction Buffer: 13mL

High Purity and Transcriptional Activity
Highly Specific Recognition of T7 Promoter
Nuclease and Protease-Free
Reduced Non-Specific Amplification
Stringent Quality Control
Manufactured in Gmp Compliant System

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