T7 RNA Polymerase, 25000U, Seafrom (CAT#: STEM-CT-0093-ZJF)

The T7 RNA Polymerase, 25000U manufactured by Seafrom is suitable Large-scale T7 RNA polymerase for high-throughput in vitro transcription, suitable for applications requiring large amounts of RNA.
T7 RNA polymerase is a DNA-dependent RNA polymerase that specifically recognizes the T7 promoter sequence (5'-TAATACGACTCACTATAGGGAGA-3'). It can catalyze the incorporation of ribonucleotide triphosphates (NTPs) into a single-stranded or double-stranded DNA template downstream of the T7 promoter, thereby synthesizing RNA complementary to the DNA template downstream of the T7 promoter. Linear plasmids containing the T7 promoter or PCR products with the T7 promoter can be used as templates for in vitro RNA synthesis.

Source: Expressed in Escherichia coli, expressing the gene for bacteriophage T7 RNA polymerase
Specification: 25,000U
Includes:
5× RNA Polymerase Reaction Buffer: 13mL
100 mM DTT: 13mL
Storage Temperature & Shelf Life: Stored at -20°C, shelf life of 24 months
Enzyme Storage Buffer:
50mM Tris-HCl (pH 7.9), 100mM NaCl, 1mM EDTA, 20mM β-mercaptoethanol, 0.1% Triton® X-100, 50% glycerol
1×RNA Polymerase Reaction Buffer Composition:
40mM Tris-HCl (pH 7.9), 10mM DTT, 6mM MgCl2, 2mM spermidine
Enzyme Activity Unit Definition:
In a 100μL system, 1 unit of enzyme is defined as the amount needed to incorporate 5 nmol of ATP into a polymeric nucleotide at 37°C within one hour
Enzyme Activity Test Conditions:
In a 50μL reaction system containing 1×RNA Polymerase Reaction Buffer, 2mM NTPs (0.5mM each of ATP, UTP, CTP, GTP), 10mM DTT, and 1μg of T7 promoter-bearing DNA template
Quality Assurance:
T7 RNA Polymerase does not contain other nucleases such as SP6 or T3 RNA polymerase, DNAases, and RNAases.

High Purity and Transcriptional Activity
Highly Specific Recognition of T7 Promoter
Nuclease and Protease-Free
Reduced Non-Specific Amplification
Stringent Quality Control
Manufactured in Gmp Compliant System

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