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Stable isotope analysis of fatty acids by gas chromatography–isotope ratio mass spectrometry (CAT#: STEM-ST-0053-LJX)

Introduction

Compound-specific isotope analysis (CSIA) of fatty acids is a relatively young analytical method. However, CSIA of fatty acids has increasingly become the method of choice in areas where accurate and precise knowledge of isotopic composition at natural abundance level is important. CSIA of fatty acids at natural abundance level provides information on biogenetic and geographic origin of lipids and oils that is invaluable for research into archaeology and the environment and almost indispensable these days for authenticity control and fraud detection in food analysis. In combination with naturally enriched or stable isotope labelled precursors, CSIA of fatty acids has also gained increasing importance in biochemical, -medical and -geochemical applications as it offers a reliable and risk-free alternative to the use of radioactive tracers.




Principle

Isotope ratio mass spectrometry (IRMS) leverages magnetic sector mass spectrometry to enable high-precision measurement of the stable isotope content of a sample. Typical measurements target hydrogen, carbon, nitrogen, and oxygen analyses—although elements with masses up to and including sulfur can be measured. Solid, liquid, or gas phase samples are converted to simple gases then introduced to the IRMS. During analysis, an electron impact source ionizes sample-derived gas which is then accelerated down a flight tube, separated by mass, and quantified using a series of Faraday cups. The high precision of IRMS enables enumeration of even very small isotopic fractionation associated with physical, chemical, and biological transformations or natural abundance measurements.

Applications

For explaining the detailed molecular mechanisms behind biological processes
For understanding and quantifying nutrient and material exchanges between ecosystems
For providing ultra-precise stable isotope analyses
For understanding the geological history of the Earth
For food authenticity, forensic science, medical research and anti-doping testing

Procedure

1. Fill the reaction tube and install it, connect the gas path
2. Check for helium leaks
3. Heat up the reactor, wait for the reaction tube to burn stable, adjust the state of the equipment
4. Wrap the sample in a tin cup and test the sample
5. Store and process data

Materials

• Sample Type:
Fatty acids

Notes

1.The approach is also valuable for quantifying the reactivity and progression of an applied stable isotope tracer to help determine reaction rates and final disposition of applied substrates.
2.IRMS offers a way of measuring isotopic variations with extremely high levels of accuracy. It can be used to detect isotope values of lighter elements with no issues, making it instrumental in the analysis of organic and natural samples.
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