Study of actin dynamics in synapses deep inside living brain slices by stimulation emission depletion microscopy (CAT#: STEM-MIT-0371-LJX)

Introduction

Actin is a class of globular multifunctional proteins that form microfilaments. It is essentially present in all eukaryotic cells and is part of the contractile apparatus in muscle cells, which is essential for cell movement and contraction.

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Principle Applications Procedure Materials Notes Related Products

Principle

Stimulated emission depletion (STED) microscopy uses two light sources. One source emits light that excites the fluorophores, and the other emits a ring laser of different wavelengths, which is used to suppress fluorescence.

Applications

Imaging of the intensity distribution of the fluorescent sample
Imaging of living samples
Measuring of the fluorescence lifetime and fluorescence correlation spectrum of the fluorescent samples
Used in the fields of biology, medicine and materials science

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Living brain slices

Notes

Operate in strict accordance with the operating procedures, and shall not arbitrarily change the operating procedures

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