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Study of FtsZ by Linear dichroism (LD) (CAT#: STEM-ST-0117-WXH)

Introduction

Flow linear dichroism (LD) spectroscopy provides information on the orientation of molecules in solution and hence on the relative orientation of parts of molecules.
FtsZ is a protein encoded by the ftsZ gene that assembles into a ring at the future site of bacterial cell division (also called the Z ring). FtsZ is found in almost all bacteria, many archaea, all chloroplasts and some mitochondria, where it is essential for cell division. FtsZ assembles the cytoskeletal scaffold of the Z ring that, along with additional proteins, constricts to divide the cell in two.




Principle

Linear dichroism (LD) or diattenuation is the difference between absorption of light polarized parallel and polarized perpendicular to an orientation axis. It is the property of a material whose transmittance depends on the orientation of linearly polarized light incident upon it. LD measurements are based on the interaction between matter and light and thus are a form of electromagnetic spectroscopy.

Applications

Study the functionality and structure of molecules.

Materials

• Polarizer
• Absorbance spectrometer
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