Study of interactions between Agrin and small molecules by Dynamic light scattering (DLS) (CAT#: STEM-MB-0543-WXH)

Introduction

Agrin is a heparin sulphate proteoglycan located in the extracellular matrix, and plays a crucial role in aggregation of neuromuscular junction and formation of synapses. It is composed of an N-terminal domain (NtA), a series of follistatin-like domains (FS), and three laminin G-like Cterminal domains (G1, G2, and G3). The C-terminal G3 domain of agrin is known to interact with calcium if the alternative amino-acid splicing site has eight amino-acid inserts present

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Principle Applications Procedure Materials Notes Related Products

Principle

Dynamic Light Scattering (DLS) is an established and precise measurement technique for characterizing particle sizes in suspensions and emulsions. It is based on the Brownian motion of particles - this states that smaller particles move faster, while larger ones move slower in a liquid. The light scattered by particles contains information on the diffusion speed and thus on the size distribution.
The Dynamic Light Scattering (DLS) technique measures motion optically by recording the scattered light signal at a fixed angle. The particles are illuminated with a monochromatic, coherent light source (laser) and the light scattered by the particles is recorded.

Applications

DLS is used to characterize the size of various particles including proteins, polymers, micelles, Protein cages and virus-like particles, vesicles, carbohydrates, nanoparticles, biological cells, and gels.

Procedure

1. Sample preparation
2. Measurement by DLS instrument
3. Data analysis

Materials

• Dynamic Light Scattering (DLS) instruments (Photon Correlation Spectroscopy or Quasi-Elastic Light Scattering instruments)
• Dynamic Light Scattering Detector

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