Study of interactions between G-quadruplexes and RHAU by Dynamic light scattering (DLS) (CAT#: STEM-MB-0545-WXH)

Introduction

RHAU (RNA Helicase associated with AU-rich element, also known as DHX36 or G4R1) is a 114-kDa human RNA helicase of the DEAH-box family of helicases encoded by the DHX36 gene. RHAU exhibits a unique ATP-dependent guanine-quadruplex (G4) resolvase activity and specificity for its substrate in vitro. RHAU binds G4-nucleic acid with sub-nanomolar affinity and unwinds G4 structures much more efficiently than double-stranded nucleic acid.

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Principle Applications Procedure Materials Notes Related Products

Principle

Dynamic Light Scattering (DLS) is an established and precise measurement technique for characterizing particle sizes in suspensions and emulsions. It is based on the Brownian motion of particles - this states that smaller particles move faster, while larger ones move slower in a liquid. The light scattered by particles contains information on the diffusion speed and thus on the size distribution.
The Dynamic Light Scattering (DLS) technique measures motion optically by recording the scattered light signal at a fixed angle. The particles are illuminated with a monochromatic, coherent light source (laser) and the light scattered by the particles is recorded.

Applications

DLS is used to characterize the size of various particles including proteins, polymers, micelles, Protein cages and virus-like particles, vesicles, carbohydrates, nanoparticles, biological cells, and gels.

Procedure

1. Sample preparation
2. Measurement by DLS instrument
3. Data analysis

Materials

• Dynamic Light Scattering (DLS) instruments (Photon Correlation Spectroscopy or Quasi-Elastic Light Scattering instruments)
• Dynamic Light Scattering Detector

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