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Study of pores of the palatine tonsillar crypt and pharyngeal tonsillar recess by scanning electron microscope technology (CAT#: STEM-MIT-0079-LJX)

Introduction

The crypt of the palatine tonsil and the recess of the human pharyngeal tonsil can be studied using a scanning electron microscope. Many pores, ranging in diameter from 10 to 100 microns, were observed on the luminal surface of the crypts. All of the crypt surface except at the sites of pores was covered by epithelial cells. Non-epithelial round cells, mostly lymphocytes, were exposed directly to the crypt lumen through these pores. In the lumen of palatine tonsillar crypts, numerous round cells were present which evidently pass through these pores from the parenchyma.




Principle

Scanning electron microscope (SEM) is another tool to study the surface morphology, which is different from transmission electron microscope and optical microscope. SEM uses extremely narrow electron beams to scan the sample and uses point-by-point imaging to obtain an enlarged image. SEM generates secondary electron emission through the interaction between the electron beam and the sample, and the secondary electron can produce the morphologic image of the sample surface enlargement. SEM can directly utilize the material properties of the sample surface for microscopic imaging.
SEM provides the possibility to study the relationship between the three-dimensional structure of cell or tissue surface and antigen composition. The markers used in scanning electron microscopy should be able to be in the range of scanning electron microscopy, and have good localization ability to cell or tissue antigen. The selection of markers should be based on the purpose of the study. If the volume of the marker cells is large, large markers should be used, while small, easily identifiable markers should be selected to locate the receptor.

Applications

Imaging and analysis in the fields of biology, medicine, materials and chemistry

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
The palatine tonsillar crypt and pharyngeal tonsillar recess

Notes

Turn off the power when the device is not in use
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