Study of ssDNA oligonucleotides binding with silver ions (Ag+) by Dual polarization interferometry (DPI) (CAT#: STEM-MB-0447-WXH)

Introduction

Silver has been used in various applications such as in food, water purification, and medical materials for a long time because it inhibits activities of many bacteria, viruses, and cancer cells. However, silver ion also does harm to the environment and the human body as a heavy metal ion. As Ag+ could bind to many metabolites and inactivate sulfhydryl enzymes, it may produce dose-dependent cytopathogenic effects in distinct cell types. Besides, Ag+ can selectively bind to cytosine−cytosine (C−C) mismatches and forms C−Ag+−C complexes in DNA duplexes, which may interfere in the replication and transcription of DNA. Thus, it is of great importance to study the interaction of Ag+ with DNA and develop highly sensitive detection method for Ag+.

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Principle Applications Procedure Materials Notes Related Products

Principle

Dual polarization interferometry (DPI) is an analytical technique that allows the simultaneous determination of thickness, density, and mass of a biological layer on a sensing waveguide surface in real time. DPI focuses laser light into two waveguides. One of these functions as the "sensing" waveguide having an exposed surface while the second one functions to maintain a reference beam. A two-dimensional interference pattern is formed in the far field by combining the light passing through the two waveguides. The DPI technique rotates the polarization of the laser, to alternately excite two polarization modes of the waveguides. Measurement of the interferogram for both polarizations allows both the refractive index and the thickness of the adsorbed layer to be calculated. These measurements can be used to infer conformational information about the molecular interactions taking place, as the molecule size (from the layer thickness) and the fold density (from the RI) change.

Applications

Study of ssDNA oligonucleotides binding with silver ions (Ag+)

Procedure

1. Setting of dual polarization interferometry
2. Preparing the DPI sensor chip
3. Immobilization of target on DPI biosensor
4. Reagent was injected to react
5. Quantitative analysis

Materials

• DPI biosensor
• DPI sensor chip

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