Study on cultured mouse skeletal myocytes by confocal laser scanning microscopy technology (CAT#: STEM-MIT-0161-LJX)

Introduction

Skeletal muscle is a type of striated muscle, the muscle attached to the bone. Skeletal muscle is made up of muscle cells arranged in bundles. The length of each cell is different, and the cells are closely arranged, and the length is complementary. Each cell is surrounded by a thin mesh membrane called the endomyces; Each muscle bundle is wrapped by a connective tissue membrane mixed with glial fibers and elastic fibers, called the muscle bundle membrane; On the outside of each muscle, there is a thick layer of connective tissue, called the epimymuscular membrane. The connective tissue of each membrane is continuous with each other, and the blood vessels and nerves distributed to the muscle enter along the connective tissue membrane. Skeletal muscle contraction is controlled by consciousness, so it is also called "voluntary muscle". Contraction is characterized by rapid and powerful, but not lasting.

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Principle Applications Procedure Materials Notes Related Products

Principle

Laser scanning confocal microscope is a high-tech microscope. It is based on fluorescence microscope imaging and equipped with a laser scanning device, which uses ultraviolet or visible light to excite the fluorescence probe, thereby obtaining fluorescence images of the internal microstructure of cells or tissues.
The laser beam is used as the light source in the laser scanning confocal microscope. The laser beam passes through the illuminating pinhole and is reflected to the objective lens through the spectroscope. The laser beam is focused on the sample, and every point on the focal plane of the specimen is scanned. If there is a fluorescent substance that can be excited in the tissue sample, the fluorescence emitted after excitation is directly reversed back to the spectroscope through the original incident light path, and is first focused when passing through the detection pinhole. The focused light is detected and collected by the photomultiplier tube (PMT), and the signal is sent to the computer, and the image is displayed on the computer monitor after processing.

Applications

Imaging and analysis in the fields of morphology, molecular cell biology, neuroscience, pharmacology, genetics

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Mouse skeletal myocytes

Notes

Operate in strict accordance with the operating procedures, and shall not arbitrarily change the operating procedures
In the starting sequence of the switch and in the scanning process, try to do fast and orderly, to protect the laser

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