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Surface Analysis of Dental Materials by UV-Vis Spectroscopy (CAT#: STEM-MB-0870-WXH)

Introduction

For centuries in dentistry, the search remains for the best methods and materials to restore missing tooth structures or replace missing teeth.
The application of the material in the oral cavity, i.e., in direct contact with tissues, places special demands on this group of biomaterials regarding their physicochemical and biological properties, i.e., biocompatibility, local and general harmlessness to the organism, resistance to the effects of physicochemical factors in the oral cavity and biophysical indifference. In addition, the accuracy in mimicking natural tooth shapes, stability of mechanical properties, ease processing, appropriate aesthetics, and finally a moderate price are further requirements that need to be met.




Principle

UV-Vis spectroscopy is an analytical technique that measures the amount of discrete wavelengths of UV or visible light that are absorbed by or transmitted through a sample in comparison to a reference or blank sample. This property is influenced by the sample composition, potentially providing information on what is in the sample and at what concentration. The only requirement is that the sample absorb in the UV-Vis region, i.e. be a chromophore. Absorption spectroscopy is complementary to fluorescence spectroscopy. Parameters of interest, besides the wavelength of measurement, are absorbance (A) or transmittance (%T) or reflectance (%R), and its change with time.

Applications

UV/Vis spectroscopy is routinely used in analytical chemistry for the quantitative determination of diverse analytes or sample, such as transition metal ions, highly conjugated organic compounds, and biological macromolecules. Spectroscopic analysis is commonly carried out in solutions but solids and gases may also be studied.

Procedure

1. Calibrate the Spectrometer
2. Perform an Absorbance Spectrum
3. Kinetics Experiments with UV-Vis Spectroscopy

Materials

UV/VIS Spectrophotometer
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